Abstract: The motion control system for wheeled robot is composed of its vision system, human-computer interface through manipulation of the mouse, wireless transmitter/receiver module, and the two-wheeled robot. The motion control algorithms are the speeds of the left and the right wheel derived from the motion model of the robot. The correspondence of the basic motion of the robot to the operation of the left key, the right key, and the wheel of the mouse is constructed, and the MFC program was developed to handle the operations of the mouse based on the message map, and the motion control program was embedded in the procedure function of the wheel operation. The flow diagram and the codes of the program and the regulating method of the motion speed of the robot are presented in detail. The system has been applied to the nondestructive.