Abstract: Software interface is an important part for human-computer interaction. Currently, software interfaces are commonly difficult to operate, thus increasing user′s operation errors and cognitive burden. A scientific and reasonable evaluation method of software interface design quality is still in research．In this article, the method of software interface evaluation based on eye-tracking technology is given. By establishing the evaluation index of eye-tracking, collecting the data of eye-moving in typical tasks and analyzing the experimental results using data analysis software, the limitation and shortage about the design of software interface are thus found. Finally, the evaluation about the usability level of software interface is realized.