Abstract: In order to balance the two requirements of light weight and good stiffness in fixture design, topology optimization and dimension optimization methods are adopted to optimize fixture design to achieve the design goal. The topology optimization model of fixture is designed based on the topology calculation result. Compared with the initial model, the first natural frequency of topology optimization model is reduced by 36.5%, but its weight is reduced by 66.7%, which indicates a significant cost-effectiveness ratio of topology optimization. Taking the reasonable design range of design parameters as constraints, the maximum first natural frequency and the minimum weight of the fixture as optimization objectives, the dimension optimization is carried out and the detailed design model is obtained. Compared with the topology optimization model, the weight of design model is reduced by 3% and its first natural frequency is increased by 6%. After that, the random vibration test of the vibration fixture is carried out. The root mean square of the power spectral density response curve at the installation point of the tested equipment is 1.17 times that of the input spectrum at most, which indicates that the vibration fixture has good dynamic transfer characteristics and the comprehensive optimization design method of the fixture is effective and feasible.